Antimicrobial activity of different filling pastes for deciduous tooth treatment

Guedes-Pinto paste is the filling material most employed in Brazil for endodontic treatment of deciduous teeth; however, the Rifocort® ointment has been removed. Thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace Rifocort® ointment with drugs of already established antimicrobial power: Nebacetin® ointment, 2% Chlorhexidine Gluconate gel, and Maxitrol® ointment. A paste composed of Iodoform, Rifocort® ointment and Camphorated Paramonochlorophenol (CPC) was employed as the gold standard (G1). The other associations were: Iodoform, Nebacetin® ointment and CPC (G2); Iodoform, 2% Chlorhexidine Digluconate gel and CPC (G3); Iodoform, Maxitrol® ointment and CPC (G4). The associations were tested for Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus oralis (S. oralis), Enterococcus faecalis (E. faecalis), Escherichia coli (E. coli), and Bacillus subtilis (B. subtilis), using the methods of dilution on solid medium – orifice agar – and broth dilution. The results were tested using statistical analysis ANOVA and Kruskal-Wallis. They showed that all the pastes had a bacteriostatic effect on all the microorganisms, without any statistically significant difference, compared with G1. S. aureus was statistically significant (multiple comparison test of Tukey), insofar as G2 and G3 presented the worst and the best performance, respectively. All associations were bactericidal for E. coli, S. aureus, S. mutans and S. oralis. Only G3 and G4 were bactericidal for E. faecalis, whereas no product was bactericidal for B. subtilis. Thus, the tested pastes have antimicrobial potential and have proved acceptable for endodontic treatment of primary teeth.

Effects of the administration of pentoxifylline and prednisolone on the evolution of portal fibrogenesis secondary to biliary obstruction in growing animals: immunohistochemical analysis of the expression of TGF-β and VEGF

OBJECTIVE: During the neonatal and infancy periods, some chronic liver diseases may lead to progressive hepatic fibrosis, which is a condition that can ultimately result in the loss of organ function and severe portal hypertension necessitating hepatic transplantation. In a previous report, pharmacological interventions were demonstrated to modulate hepatic fibrosis induced by bile duct ligation in young rats. The administration of pentoxifylline or prednisolone, or the combination of both, resulted in reduced fibrogenesis in portal spaces. The objectives of the present study were to evaluate the expression of transforming growth factor β and vascular endothelial growth factor after bile duct ligation in young rats and to assess the effect of those same drugs on cytokine expression. METHODS: In this experimental study, 80 young rats (21 or 22 days old) were submitted either to laparotomy and common bile duct ligation or to sham surgery. The animals were allocated into four groups according to surgical procedure, and the following treatments were administered: (1) common bile duct ligation + distilled water, (2) sham surgery + distilled water, (3) common bile duct ligation + pentoxifylline, or (4) common bile duct ligation + prednisolone. After 30 days, a hepatic fragment was collected from each animal for immunohistochemical analysis using monoclonal antibodies against transforming growth factor β and vascular endothelial growth factor. Digital morphometric and statistical analyses were performed. RESULTS: The administration of pentoxifylline reduced the transforming growth factor β-marked area and the amount of transforming growth factor β expressed in liver tissue. This effect was not observed after the administration of prednisolone. There was a significant reduction in vascular endothelial growth factor expression after the administration of either drug compared with the non-treatment group. CONCLUSIONS: The administration of pentoxifylline to cholestatic young rats resulted in the diminished expression of transforming growth factor β and vascular endothelial growth factor in liver tissue. The administration of steroids resulted in the diminished expression of vascular endothelial growth factor only. These pathways may be involved in hepatic fibrogenesis in young rats submitted to bile duct ligation and exposed to pentoxifylline or prednisolone.

Effects of steroid treatment on bone mineral metabolism in children with Glucocorticoid-sensitive Nephrotic Syndrome / Efectos del tratamiento de esteroides sobre el metabolismo mineral del hueso en niños con Síndrome Nefrótico Sensible a los Glucocorticoides

Glucocorticoids have been used in nephrotic syndrome (NS) treatment for many years. In this study, we aimed to evaluate the effect of steroids on bone mineralization in children with glucocorticoid-sensitive nephrotic syndrome (GSNS). Twenty children who were first diagnosed as GSNS received glucocorticoid therapy for four months. Before treatment, at the 4th and 12th week of initial therapy, bone mineral density (BMD) and levels of the markers for bone turnover were evaluated. At the 4th and 12th week of treatment, mean serum calcium (Ca) and osteocalcin levels were found to be significantly lower than those at the beginning ofthe therapy. Mean serum total alkaline phosphatase (t-ALP), bone-specific alkaline phosphatase (b-ALP) and urine calcium creatinine ratio (Ca/Cr), urinary deoxypyridinoline levels were significantly increased in comparison to the beginning of therapy. There was no significant relationship between serum levels of phosphate and parathyroid hormone (PTH) at the beginning of treatment and at the 4th and 12th week of treatment. Mean value of BMD was significantly lower at the 4th and 12th week of treatment than that at the beginning of the therapy. In conclusion, bone mineralization was negatively affected by steroid treatment in children with NS. These children should undergo regular BMD evaluation, and an appropriate therapeutic approach should be planned.

Por muchos años se han venido usando glucocorticoides en el tratamiento del síndrome nefrótico (SN). Este estudio se encamina a evaluar el efecto de los esteroides sobre la mineralización ósea en niños con síndrome nefrótico sensible a los glucocorticoides (SNSG). Veinte niños que fueron diagnosticados primeramente con SNSG, recibieron terapia con glucocorticoides durante cuatro meses. Antes del tratamiento, en las semanas 4 y 12 de la terapia inicial, se evaluaron la densidad mineral ósea (DMO) y los niveles de los marcadores del recambio óseo. En el tratamiento de las semanas 4 y 12, se halló que el calcio (Ca) sérico promedio y los niveles de osteocalcina eran significativamente más bajos que los existentes a comienzos de la terapia. Los niveles de fosfatasa alcalina sérica total promedio, fosfatasa alcalina (t-ALP), fosfatasa alcalina especifica ósea media (b-ALP), la relación calcio/creatinina en la orina (Ca/Cr), y los niveles de deoxipiridinolina urinaria, aumentaron significativamente en comparación con los existentes al comienzo de la terapia. No hubo relación significativa alguna entre los niveles séricos de fosfato y hormona paratiroidea (PTH) ni al principio del tratamiento ni en las semanas 4 y 12 de tratamiento. El valor promedio de la DMO fue significativamente más bajo en las semanas 4 y 12 de tratamiento que al principio de la terapia. En conclusión, la mineralización del hueso fue afectada negativamente por el tratamiento con esteroides en los niños con SN. Estos niños deben tener una evaluación regular de DMO, para lo cual es necesario planear un enfoque terapéutico apropiado.

effect of prednisolone on the sensitivity of Technetium 99m-methylene diphosphonate [[99m] Tc-MDP] scintigraphy to detect simulated closed fracture in the rat tibia

The purpose of the present study was to evaluate the effect of prednisolone on the sensitivity of bone scanning to detect the simulated closed fracture in the rat tibia. A total number of forty eight adult, male NMRI rats randomly assigned into two parts, one part for 4 and other for 8 weeks experiments. Each part has been divided into four groups, one group not receiving prednisolone [control group] and the other groups receiving 5, 10 and 20 mg/kg prednisolone respectively. After four and eight weeks of experiments a simulated closed fracture was created in the tibia approximately 1 cm near knee joint. .The scintigraphy imaging has been performed on second and fifth day after tibial bone fracture and the ratio of the activity of tibia bone fracture to the contra lateral healthy side was calculated [R factor]. All scintigraphy images showed the rat tibia bone fracture. Statistically significant difference in the R factors have been observed, when scintigraphy images were performed in fifth day after injury. Bone radionuclide scanning by [99m]Tc-MDP is very sensitive for detection of occult fracture. In order to enhance bone scan sensitivity, it may be reasonable to postpone [99m]Tc-MDP scintigraphy imaging at least 2 to 3 days after suspected trauma in patients receiving glucocorticoid medication

Histological evaluation of bone response to pediatric endodontic pastes: an experimental study in guinea pig

This study aimed to evaluate by the intra-osseous implant technique the most commonly used materials for pulp therapy in pediatric dentistry: calcium hydroxide (CH), Guedes Pinto paste and CTZ paste, according to FDI (1980) and ANSI/ADA (1982) recommendations. Thirty guinea pigs, 10 for each material, divided into experimental periods of 4 and 12 weeks received one implant on each side of the lower jaw symphysis. The external lateral tube wall served as control for the technique. At the end of the observation periods, the animals were euthanized and specimens were prepared for routine histological examination. It was observed that CH and CTZ paste induced severe inflammation, a large amount of necrotic tissue, lymphocytes, foreign body cells and bone resorption, while Guedes Pinto Paste induced little or no inflammation in the 4-week observation period. After 12 weeks, the reactions to CH and Guedes Pinto paste were also absent/mild, presenting a general pattern of replacement by recently formed bone tissue while a moderate to severe inflammatory response was observed with CTZ paste. Guedes Pinto paste presented acceptable biocompatibility levels in both analyzed periods; CH only showed acceptable biocompatibility in the 12-week period while CTZ paste showed no biocompatibility in both periods. Among the tested materials, only Guedes Pinto paste presented an acceptable biocompatibility.

A pesquisa teve como objetivo avaliar a biocompatibilidade através da técnica de implantes intra-ósseos dos materiais utilizados em odontopediatria para tratamento pulpar: hidróxido de cálcio, pastas Guedes Pinto e CTZ, de acordo com as recomendações da FDI (1980) e ANSI/ADA(1982). Trinta guinea pigs, dez para cada material, divididos em períodos experimentais de 4 e 12 semanas receberam um implante em cada lado da sínfise mandibular. A parede lateral externa do copo serviu como controle para a técnica. No final dos períodos experimentais, os animais foram sacrificados e os espécimes preparados para o exame histológico de rotina. Observou-se que o hidróxido de cálcio e a pasta CTZ mostraram reação inflamatória severa, grande quantidade de tecido necrosado, linfócitos, células de corpo estranho e reabsorção óssea; enquanto a pasta Guedes Pinto induziu pouca ou nenhuma inflamação no período de 4 semanas. Após 12 semanas as reações para o hidróxido de cálcio e pasta Guedes Pinto foram ausentes/suaves apresentando um padrão geral de substituição por tecido ósseo neoformado, enquanto uma resposta inflamatória de moderada a severa foi observada para a pasta CTZ. A pasta Guedes Pinto apresentou níveis aceitáveis de biocompatibilidade nos dois períodos analisados; hidróxido de cálcio apresentou biocompatibilidade aceitável somente no período de 12 semanas e a pasta CTZ não mostrou biocompatibilidade em ambos os períodos. Entre estes, apenas a pasta Guedes Pinto apresentou níveis de biocompatibilidade nos dois períodos analisados.

comparative study of the effect of Budesonide versus prednisolone on adrenal gland of the male rabbit

Although oral glucocorticoids are the treatment of choice for moderate to severe pancolitis, their systemic side effects and adrenal suppression account for considerable morbidity. Budesonide is a new intestinal topical active glucocorticoid which displays high therapeutic efficacy and high systemic tolerability. To deliver the active drug to ileal and ileocecal area, Budesonide has been formulated into enterocapsule preparation. Several studies compared the efficacy of Budesonide with that of Prednisolone. However, few determined the extent of adrenocortical suppression occurring with both drugs on histological basis. In this work, fifteen adult male New Zealand albino rabbits were used. They were classified into three equal groups. Group I served as control. Group II included animals that received intragastrically one tablet of 5 mg prednisolone daily for four weeks. Group III included animals that received orally one capsule of entocort containing 3 mg budesonide every other day for four weeks. The adrenals were processed for histological and immunohistochemical study. In the present comparative study, evidence of adrenal suppression was significantly greater in the prednisolone group than in budesonide treated animals. Light microscopic examination of H and E stained sections of prednisolone group, revealed an apparent decrease in the size of zona fasciulata cells which was proved significant by morphometric study. Moreover, Oil red stained sections of group II demonstrated a relative decrease in cytoplasmic lipid content of both zona glomerulosa and fasciculata. Using chromaffin reaction, it was noticed that there was a relative increase in the number of nor-epinephrine cells than in group III. Immunohistochemical study showed that most of the nuclei of cells in zona fasciculata in group II were negatively stained for proliferating cell nuclear antigen [PCNA] which was further proved significant morphometrically using the PCNA index. Thus it was concluded that, the budesonide preparation is associated with much less impairment of adrenal axis function. Therefore, budesonide offers a useful advance in treatment of inflammatory bowel disease

Improvement in sperm motility viability and normality after treatment with prednisolone, antisperm antibody separation and in vitro sperm activation in immunologically infertile men

Antisperm antibody [ASA] as a cause of men infertility was fist reported in 1954. Different assays were developed for the detection of scrum-bound antibodies. The objective of this study is to study the effect of prednisolone, antisperm antibody separation [ASAS] and in vitro sperm activation on sperm motility, viability and morphology in immunologically infertile patients. Semen samples of 250 immunologically infertile patients were examined by seminal fluid analysis and microagglutination test to check the presence of sperm agglutination and antisperm antibodies [ASA]. The patients received 5mg prednisolone three times per day for two weeks then the dose was reduced to two tablets per day for four days and further reduced to one tablet for three days followed by one week of rest period. The treatment regimen was continued for three months and seminal fluid analysis was performed before and after the treatment. Sperm motility percent, sperm grade activity, sperm motility index, normal sperm morphology and sperm viability after the treatment were significantly improved [P< 0.001] while the percent of sperm agglutination, shaky head sperm movement percent of abnormal sperm morphology were significantly decreased [P < 0.0001]. the progress in the improvement of sperm quality was started at the end of 4[th] week after treatment and increased gradually up to the end of the 12[th] week. Application of prednisolone therapy, ASAS technique and in vitro sperm activation found to be effective and resulted in significant improvement sperm quality. These active and viable sperm following treatment may be used for intrauterine insemination or in vitro fertilization [IVF] and embryo transfer [ET] for the treatment of immunological infertile men

Effects of anti-allergic drugs on intestinal mastocytosis and worm expulsion of rats infected with Neodiplostomum seoulense

The effects of anti-allergic drugs on intestinal mastocytosis and the expulsion of Neodiplostomum seoulense were observed in Sprague-Dawley rats, after oral infection with 500 metacercariae. The drugs used were hydroxyzine (a histamine receptor H1 blocker), cimetidine (a H2 blocker), cyclosporin-A (a helper T-cell suppressant), and prednisolone (a T- and B-cell suppressant). Infected, but untreated controls, and uninfected controls, were prepared. Worm recovery rate and intestinal mastocytosis were measured on weeks 1, 2, 3, 5, and 7 post-infection. Compared with the infected controls, worm expulsion was significantly (P < 0.05) delayed in hydroxyzine- and cimetidine-treated rats, despite mastocytosis being equally marked in the duodenum of all three groups. In the cyclosporin-A- and prednisolone-treated groups, mastocytosis was suppressed, but worm expulsion was only slightly delayed, without statistical significance. Our results suggest that binding of histamine to its receptors on intestinal smooth muscles is more important in terms of the expulsion of N. seoulense from rats than the levels of histamine alone, or mastocytosis.

In vitro resistance of leukaemic blasts to prednisolone in bcr-abl positive childhood acute lymphoblastic leukaemia.

BACKGROUND & OBJECTIVES: Although the outcome of children with acute lymphoblastic leukaemia (ALL) has improved dramatically over the last decade, some children still fare poorly and relapses are seen. The sensitivity of leukaemic cells to corticosteroids has emerged as an important prognostic factor in ALL. The t(9,22) translocation, resulting in the bcr-abl fusion gene, is a non-random translocation found in B-lineage acute lymphoblastic leukaemia. It is also known to be an independent poor prognostic factor for long-term disease free survival. We studied the association between the presence of bcr-abl fusion gene and in vitro prednisolone resistance in children with B-lineage acute lymphoblastic leukaemia at diagnosis. METHODS: A total of 23 children (aged 1-16 yr, median age: 12 yr) with B-lineage acute lymphoblastic leukaemia at diagnosis were included in the study. The presence of bcr-abl fusion gene was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and the in-vitro resistance to prednisolone was measured by short term colorimetric methyl thiazol tetrazolium (MTT) assay. RESULTS: A median LD50 (lethal dose for 50% cells) for prednisolone in bcr-abl positive children (n=7) was 1.6 mg/ml (range: 0.25-5.0 mg/ml) and that of bcr-abl negative children (n=16) was 0.35 mg/ml (range 0.62-1.0 mg/ml). The median LD50 for prednisolone differed significantly between the bcr-abl positive and negative groups of children with acute lymphoblastic leukaemia (P<0.005). INTERPRETATION & CONCLUSION: This is probably the first report to show that leukaemic blasts of bcr-abl positive children with ALL are about four-fold resistant to prednisolone as compared to blasts from bcr-abl negative children. This suggests that one of the reasons for the poor prognosis of bcr-abl positive ALL could be a lower steroid sensitivity.

Drug sensitivity assay for leukaemic cells by flow cytometry.

BACKGROUND & OBJECTIVES: Drug sensitivity assays are useful in oncology practice for evaluating the sensitivity of malignant cells to anti-cancer drugs. The usefulness of such assays for the prediction of clinical response to therapy has also been demonstrated. The existing methods used for this purpose are time consuming and labour intensive. Here we report a simplified flow cytometry based assay for evaluating the in vitro drug sensitivity of leukaemic cells. METHODS: The chemo-sensitivity of three human leukaemic cell lines (a lymphoblastoid cell line, Jurkat; an erythroleukaemic cell line, K 562 and a myelomonocytic cell line HL-60) was investigated by flow cytometry. Flow cytometry was used to determine LD50 (50% inhibitory concentration) for prednisolone on Jurkat and daunorubicin on HL 60 and K 562 cell lines respectively. Per cent cell death could directly be assessed on a flow cytometer by measuring the fluorescence after staining with propidium iodide (PI). For comparison MTT assay was also performed using prednisolone on Jurkat and daunorubicin on HL-60. RESULTS: Cytotoxic effect of drugs was found to be dose dependent. Mean LD50 of prednisolone for Jurkat cells by flow cytometry was 0.805 +/- 0.058 mg/ml and by MTT assay 0.866 +/- 0.115 mg/ml. Mean LD50 of daunorubicin for HL-60 was 1.96 +/- 0.05 micrograms/ml by flow cytometry and 1.90 +/- 0.282 micrograms/ml by MTT assay. The mean LD50 of daunorubicin to K 562 was 0.49 +/- 0.049 mg/ml by the flow cytometry method. The inter-assay variation for the LD50 by flow cytometry based assay was found to be 6, 14 and 10 per cent for Jurkat, HL-60 and K 562 respectively. INTERPRETATION & CONCLUSION: We report a flow cytometry based drug-sensitivity assay for leukaemic cells, which uses a single dye staining and is rapid, technically simple and reproducible. The results compare well with the more commonly used MTT assay, which is labour intensive and time consuming. The limitation of our method is that it can only be used for studying cells in suspension and is therefore not suitable for adherent cell lines.

Prednisolone in Duchenne muscular dystrophy.

This Study was carried out in the department of Neuromedicine, Bangabandbu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. A total of 19 Duchenne muscular dystrophy (DMD) patients were recruited for randomized controlled study to see the effect of prednisolone in the natural course of the disease process. Prednisolone was given in 10 patients (study group) in a dose of 0.75 mg/kg for six months. Vitamin was given in 8 patients (control group) for the same duration. One patient was dropped from the study. Patients were assessed for average muscle strength, timed function test and functional grades (pelvic and pectoral). It was found that at the end of the study, almost all the parameters were improved significantly in the prednisolone treated group (P < 0.05).

Effect of herbal preparation on immune response of immunosuppressed mice.

The present study was undertaken to study the effect of a herbal formulation (Septilin) as immunomodulator, on immune response in mice. The study of this formulation in respect to humoral and cell mediated immune response has suggested that, oral administration of Septilin (500 mg/kg) alone or in combination with an immuno-suppressive drug (prednisolone 4 mg/kg), enhances both primary and secondary immune response, in mice immunized with sleep red blood cells (SRBC).

Efecto inhibitorio de ciclosporina y prednisona sobre la fibrosis pulmonar por bleomicina en la rata / Inhibitory effect of cyclosporin-A and prednisone on bleomycin-induced pulmonary fibrosis in the rat

La administración intratraqueal de bleomicina (BLM) induce una fibrosis pulmonar en la rata detectable al cabo de 14 días. Tanto la ciclosporina-A como la Prednisona (administrada contemporáneamente con la bleomicina y luego diariamente hasta 14 ó 30 días después de BLM) disminuyen en cerca del 50 por ciento la fibrosis pulmonar inducida por bleomicina (Puntaje histopatológico). El Própósito de este estudio fue determinar si la ciclosporina y la prednisona administradas una vez que la fibrosís pulmonar estaba ya establecida eran capaces de inhíbirla. 1 U de BLM/100 g de peso corporal fue administrada intratraquealmente y las ratas se sacrificaron 14 y 30 días después. Se inyectó ciclosporina (0,15 mg/lOO g/día, ip) y/o prednisona (0,1 mg/1OO g/día, ip) desde el día 14 al 30 post-BLM. Prednisona o ciclosporina disminuyeron en cerca del 50 por ciento la fíbrosis pulmonar inducida por BLM. Sin embargo, la asociación prednisona + ciclosporina redujo la fíbrosis en sólo 40 por ciento. La disminución de la fibrosis pulmonar estuvo asociada a cambios en el líquido del lavado broncoalveolar: recuento celular, contenido de proteínas y fosfolípídos y actividad gama glutamil transpeptídasa. Se concluye que la prednisona y la ciclosporina son capaces de disminuir una fibrosís pulmonar ya establecida inducida por BLM en la rata

Effect of calcium on basal and stimulated gastric secretion

The role of extracellular calcium in the control of basal and stimulated gastric secretion was studied by using the Ca[++] channel blocker, verapamil. The volume of gastric secretion [in ml], free and total acidity [as unit%] and peptic activity [as unit/ml] were the parameters used to assess gastric function. All the drugs were given intraperitoneally every day for four successive days. The results demonstrated a stimulatory effect of prednisolone [25 mg/kg] and histamine [1 mg/kg] on gastric secretion. Verapamil [7 mg/kg] resulted in significant decrease in basal gastric secretion. Still, verapamil inhibited the stimulatory effect of prednisolone and histamine on basal gastric secretion. This inhibition was significantly more on prednisolone than histamine simulated gastric secretion, an interesting finding that needs further clarification. The mechanism of the inhibitory effect of verapamil could be through inhibiting the action of H[+] - K[+] ATPase enzyme or due to inhibiting transmembrane flux of Ca[++] ion which is necessary for the process of gastric secretion

Estudio comparativo in vitro de tabletas perorales conteniendo prednisona / Comparative study in vitro of peroral tablets containing prednisone

En el presente trabajo, se ha sometido a comparación fisicoquímica a tabletas de administración peroral conteniendo prednisona 5mg. como principio activo, comercializada actualmente en el Perú. Además se hace una comparación entre el medio de disolución exigido por la farmacopea, agua, con jugo gástrico. Los ensayos se han realizado de acuerdo a exigencias de la USP XXII. De los cinco productos analizados se demuestra que los correspondientes a la clave B, C, D y E son potencialmente equivalentes por presentar homogenicidad en la disolución de lote y en la uniformidad de contenido. El producto A no cumple con las especificaciones requeridas. No se encontró significado estadístico entre las diferencias de los valores medios de agua y jugo gástricos para cada producto respectivamente, lo cual indica que los resultados dados para ambos casos son válidos. Se hace incapié sobre la importancia de la biodisponibilidad de los medicamentos y la necesidad de efectuar el ensayo de devolución a éstos para garantizar su biodisponibilidad.

Alteration in the levels of pyrazinamide in pleural fluid following simultaneous administration of prednisoline in patients of tubercular pleural effusion.

20 Patients of tuberculous pleural effusion were administered a combination of pyrazinamide (30 mg/kg) + isoniazid (300 mg) orally for 7 consecutive days and pyrazinamide was estimated by spectrophotometric method in serum and pleural fluid. Prednisolone was added to the above regimen for next 7 consecutive days and pyrazinamide was again estimated. The level of pyrazinamide in pleural fluid was 23.4 +/- 1.2 (micrograms/ml). Following addition of prednisolone the level increased (27.6 +/- 1.3) significantly (P less than 0.001). The serum pyrazinamide level was not influenced by simultaneous administration of prednisolone. The pleural fluid/serum pyrazinamide ratio was increased from 0.465 to 0.542 by the addition of prednisolone to therapeutic regimen.

Effects of prednisolone and dexamethasone on (pro)insulin biosynthesis and insulin release by rat islets of Langerhans.

Administration of either prednisolone or dexamethasone (10 mg/kg body wt/day/oral) to rats for 21 days resulted in inhibition of (pro)insulin biosynthesis and immunoreactive insulin release by isolated islets. A gradual reversal of prednisolone's effect was obtained after exposing islets to increasing glucose concentrations but glucose challenges failed to influence dexamethasone's effect.

Desarrollo de una formulación utilizando ensayos acelerados: estabilidad de la prednisolona en atomical con prednisolona / Development of a formulation using accelerated assays: stability of prednisolone in prednisolone-containing atomycol

Se realiza el estudio de la estabilidad de una formulación presente en el mercado (prednisolona) y se llega a la conclusión de que la misma posee baja estabilidad. Se preparan varios pares en los cuales se encuentra la prednisolona y otro de uno de los constituyentes de la formulación, incluyendo el metabisulfito de sodio. Estas soluciones se degradaron de acuerdo con una ecuación de primer orden. Se demuestra que el propionato de sodio, como agente antimicótico, no es el idóneo para la formulación y que el metabisulfito debe ser sustituido por otro agente antioxidante